SECTION 1
Biochemical Investigation, Buffers, Microscopy & Centrifugation
Covers the introductory and foundational part of Bioinstrumentation.
This section introduces physiological solutions and buffers,
approaches used in biochemical investigations, methods of
cell disruption and protein extraction, microscopy techniques,
and centrifugation techniques.
- Buffers and Physiological Solutions
- General Principles and Approaches of Biochemical Investigations
- Cell Disruption and Extraction
- Protein Source Selection and Solubilization
- Phase-Contrast Microscopy
- Electron Microscopy
- Fluorescence Microscopy
- Centrifugation Techniques
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SECTION 2
Electrophoretic & Chromatographic Techniques
Major separation techniques used in biochemical and molecular investigations.
This section focuses on electrophoretic methods and the major
chromatographic techniques used for separation, identification,
purification and analysis of biological molecules.
- Electrophoretic Techniques
- Agarose Gel Electrophoresis
- Polyacrylamide Gel Electrophoresis
- Isoelectric Focusing
- Two-Dimensional Electrophoresis
- Principles of Chromatography
- Ion-Exchange Chromatography
- Affinity Chromatography
- Paper Chromatography
- Thin-Layer Chromatography
- Gel Permeation Chromatography
- Gas Chromatography
- High Performance Liquid Chromatography (HPLC)
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SECTION 3
Spectroscopy & Molecular Techniques
Instrumental methods for spectroscopic analysis and molecular investigation.
This section covers important spectroscopic techniques and
molecular biology instruments used for characterization,
detection and analysis of biological molecules.
- Lambert–Beer Law
- X-Ray and Gamma-Ray Spectroscopy
- Spectrofluorometry
- Optical Rotation Dispersion
- Electron Spin Resonance (ESR)
- Infrared Spectroscopy
- Nuclear Magnetic Resonance Spectroscopy
- Mass Spectrometry
- Ultraviolet and Visible Spectroscopy
- Thermocycler
- Probes
- Sequencer
- Gel Documentation System
- Microarray
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