Systemic & Diagnostic Virology-I

Detailed, syllabus-based notes covering diagnostic virology and medically important viruses for MSc Medical Microbiology Semester II.

Exam-oriented questions and question-bank material designed according to the MB 558 syllabus.

Course Focus

Diagnostic Virology: cultivation and purification of viruses, serodiagnostic methods, nucleic-acid based diagnosis and microscopic techniques.
Serological Methods: haemagglutination, haemagglutination inhibition, complement fixation, neutralization, Western blot, RIPA, flow cytometry and immunohistochemistry.
Molecular Diagnosis: nucleic acid hybridization, PCR, microarray and nucleotide sequencing.
Specimen Management: selection, collection, transportation and processing of clinical specimens for laboratory diagnosis of viral infections.
Poxviruses: Smallpox virus, including structure, pathogenesis, medical importance, laboratory diagnosis and control.
Herpesviruses: Herpes simplex virus, Varicella-zoster virus, Cytomegalovirus, HHV-6, HHV-7 and Epstein-Barr virus.
Picornaviruses: classification, structure, pathogenesis, medical importance and laboratory diagnosis.
Orthomyxoviruses: Influenza virus and its laboratory diagnosis, pathogenesis and control.
Paramyxoviruses: Measles virus, Mumps virus, Human parainfluenza viruses and Human respiratory syncytial virus.
Arboviruses: Dengue virus, Japanese encephalitis virus and Yellow fever virus.
Retroviruses: HIV and HTLV, including their structure, pathogenesis, medical importance and laboratory diagnosis.

Prepare each virus with emphasis on its structure, classification, pathogenesis, clinical importance, laboratory diagnosis and prevention/control.

2 marks: definitions, classification, important properties, examples and short diagnostic methods.
5 marks: individual diagnostic methods, pathogenesis, virulence/clinical features and short notes on medically important viruses.
10 marks: complete discussion of important viruses including structure, pathogenesis, clinical features, laboratory diagnosis and prevention/control.

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