This virtual practical simulates the routine laboratory examination of a sputum specimen, including specimen assessment, macroscopic examination, selected chemical observation, direct microscopy, Gram staining, culture and appropriate pathogen confirmation.
The specimen is randomized every time the practical is restarted. Therefore, the appearance, microscopic findings and final interpretation can change.
Important branching rule: once a bacterial respiratory pathogen is confirmed, the practical proceeds through the bacteriology pathway and does not enter the non-bacterial confirmation pathway. When a fungal/non-bacterial finding is confirmed, the practical does not proceed to bacterial confirmation.
Observations will appear here as the sputum specimen is processed.
Select the correct material or reagent from its rack. Correct selections will physically move the object to the appropriate workbench area.
Randomized specimen: Loading...
Branch status: Not yet determined.
| Specimen quality | Pending |
|---|---|
| Macroscopic examination | Pending |
| Microscopy | Pending |
| Gram stain | Not yet performed |
| Bacterial culture | Not yet performed |
| Non-bacterial examination | Not yet performed |