Antibiotic Susceptibility Testing Virtual Laboratory
Perform antimicrobial susceptibility testing using standardized laboratory procedures. Work through each practical step by step using the laboratory equipment on the workbench.
Practical Overview
Antibiotic susceptibility testing determines how a bacterial isolate responds to antimicrobial agents. In this virtual laboratory, the student performs the major practical actions rather than simply reading the procedure.
- Standardized bacterial inoculum
- 0.5 McFarland turbidity comparison
- Mueller–Hinton agar
- Lawn culture preparation
- Kirby–Bauer disc diffusion
- Agar well diffusion
- Incubation and zone formation
- Zone diameter measurement
- Susceptible, intermediate and resistant interpretation
- MIC, MBC and E-test concepts
Materials Rack
CULTURE MATERIAL
Bacterial
Suspension
Suspension
0.5 McFarland
SWABBING
Sterile Swab
Sterile Forceps
PLATES
Agar Plate
WELL DIFFUSION
Cork Borer
Dropper
MEASUREMENT
Zone Ruler
Antibiotic / Test Material Rack
ANTIBIOTIC DISCS
AMC
Amoxicillin/
Clavulanate
Clavulanate
CIP
Ciprofloxacin
GEN
Gentamicin
AMP
Ampicillin
E-TEST
E-TEST
MIC Strip
Main Workbench — Practical Work Area
Perform the current laboratory operation here.
0.5 McFarland Turbidity Comparison
Turbidity matches the 0.5 McFarland reference.
Incubation Station
READY
Sterilization Station
Bunsen Burner
Biohazard Waste Station
☣
BIOHAZARD
Observation
Perform the practical to observe the result.